Mitra Khademosharie; Mohammadreza Hamedinia; Teyebeh Amiri Parsa; Seyed Alireza Hosseini Kakh
Volume 18, Issue 4 , January and February 2012, , Pages 260-271
Abstract
Background and Purpose: Leptin is one of the most important proteins involved in lipid metabolism and energy homeostasis but the effect of acute exercise on leptin has been less investigated. The purpose of the present study was to investigate the effect of a one-session exercise on tissue concentration ...
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Background and Purpose: Leptin is one of the most important proteins involved in lipid metabolism and energy homeostasis but the effect of acute exercise on leptin has been less investigated. The purpose of the present study was to investigate the effect of a one-session exercise on tissue concentration and gene expression of leptin in rats.
Methods and Materials: In this experimental study، 24 male Wistar rats were housed in a standard environment، and were randomly divided into two groups (Control and Experimental). The exercise was running on a treadmill for 120 min (18 m/min). Immediately، 2 and 24 hours after exercise the rats anesthetized، blood، fat tissue and soleus were taken. Leptin concentration in the muscular، fat and blood were measured. Also، leptin gene expression was investigated with RT-PCR in tissues. Data were analyzed in SPSS 16 using repeated measures ANOVA (P0.05).
Conclusion: Acute exercise can increase leptin gene expression in muscle and fat tissues. However، exercise with longer duration and higher volume may appear to be more effective.